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Title   Vitamin E 가 Dimethylhydrazine 에 의한 장암 발생에 미치는 영향에 대한 연구 ( The Effect of Vitamine E on Intestinal Tumorigenesis by 1 , 2 - dimethylhydrazine )
Publicationinfo   1991 Jan; 023(01): 29-67.
Key_word   Rat Intestinal tumorigenesis model, 1, 2 dimethylhydrazine, Vitamin E effect
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Abstract   In an attempt to elucidate the effects of Vitamin E on intestinal tumorigenesis by 1, 2- dimethylhydrasine (DMH), the present study was undertaken in male Sprague-Dawley rats. Carcinogenesis was induced in male Sprague-Dawley rats with DMH 20 mg/kg or 40 mg/kg administered subcutaneously at weekly intervals for 24 doses. Vitamin E (tocopheryl acetate) was given orally to one group of DMH 20mg treated rats. All animals were sacrified when moribund or at 28th week. All organs were examined macroscopically and fixed in 10% buffered formalin. Histologic studies were routinely done on the liver, spleen, kidney, bladder, thyroid gland, heart, lungs, pancreas, duodenum, jejunum, ileum, cecum, colon, rectum, and regional nodes. The results were as follows: l. Of the DMH induced bowel neoplasms, all (100%) developed colonic tumors, and some developed rectal, cecal and smafl inestinal tumors. 2. Histologically, the tumors were classifed as polypoid adenoma, and adenocarcinoma, the latter revealed some evidence of mucin secretion. 3. The total number of tumors in the DMH 40 mg treated group was higher than that of the DMH 20 mg treated group. This difference was highly significant (p < 0.0065). 4. The degree of tumor invasion in the DMH 20 rng treated group was relatively confined to the submucosa, whereas that of the 40 mg DMH treated group was confined to the serosa, except for wide dissemination in 3 rats. 5. The total number of tumors or the degree of tumor invasion between the DMH 20 mg treated group and the DMH 20 mg+vitamin E treated group was not signifirant

0.8). In summary, the results obtained by the present study support the following theory; The metabolic material (MAM) of DMH is delivered to colon via the blood stream. MAM then breaks down either the colon lumen or colon mucosal cells to fonn highly reactive methyl diazonium and methyl carbonium ions, which are believed to be the ultimate carcinogen moieties. The present study is in contrast with the reported inhlbitory effect of Vitamin E on colon carcinogenesis.

저 자   이강혁(Kang Hyuk Lee),강대영(Dae Young Kang)